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Related post: 387 4-4 Proteins from a large variety of sources have been sequenced in the facility. These include TGFfj-related proteins (A. Duphaston Price Roberts, NCI); vaccinia virus polymerases (P. Gershon and B. Ahn, LVD); HSVI polymerase fractions (M. Chalberg, LVD); a vaccinia membrane protease (J. Keck, LVD); tumor specific antigens (B. Seon, Roswell Park); lymphocyte activation antigens (M. Sitkovsky, Duphaston 10 Mg LI); and a serum class I associated protein (D. Margulies, LI). FLOW CYTOMETRY ANALYSIS Over the past year the FCS has accomplished the following: The FCS computers are now connected, via ethernet (using LAN cabling), to both IBM PC-compatible and Macintosh PC's for analysis of data from Buildings 4,7 and 10. A new MicroVax 3000 with two large capacity disk drives, has been installed which serves as the network router and will also serve as a data analysis station. PC based analysis software, including a Coulter workstation, are being evaluated for analysis of data from all FCS instruments. The computer processor of the user operated Epics Profile has been upgraded to greatly enhance analysis and acquisition of data. 4-5 Biological Resources Branch Annual Report October 1, 1989 to September 30, 1990 ADMINISTRATIVE REPORT This period marks the third year of the existence of the Biological Resources Branch. There are four Sections within the Branch; three of which are primarily involved with providing technological resources to the NIAID intramural laboratories. In the Molecular Structure Section, Mark Garfield continues to be responsible for protein sequence analyses and during the past year has taken on the additional responsibility of making about 25% of the synthetic peptides produced within the Branch. The Tablet Duphaston Flow Cytometry Section continues to flourish under the able direction of Dr. Kevin Holmes and Mr. David Stephany. The turmoil in the production of synthetic peptides created by Duphaston 10mg Tablet the departure of Dr. W. Lee Maloy last year has subsided and the Branch produced a record number of peptides during the past year. Dr. Coligan assumed overall responsibility for the production of peptides both by the Branch itself and by the PRI contract. On the research side, Dr. Ken Parker has been hired as a Senior Staff Fellow in the Synthetic Peptide Antigen Section to work on problems in structural biology. In particular, his immediate interest center around defining the physico-chemical interaction between MHC class I molecules and peptide. A new technician, Douglas Markert, hired for the Section has since departed and recruitment is underway. In regard to postdoctoral fellows, Dr. Gregory Einhorn has departed for a position at the Research Institute of Scripps Clinic, Dr. Thomas McConnell has assumed an Assistant Professorship at East Carolina University and Dr. Kevin Moulder departed for a Duphaston Tablet position at Smith Kline Beecham, Epsom, England. Dr. Antonello Punterieri joined the laboratory as a Visiting Fellow from Dr. Hiroshi Taniuchi's lab in Buy Duphaston the NTDDK, Dr. Mark Halvorson has arrived from the Department of Microbiology, Indiana University to assume an IRTA position, and Dr. Chiara Bovolenta joined the laboratory as a Guest Researcher from the University of Verona, Italy. 4-6 Biological Resources Branch Annual Report October 1, 1989 to September 30, 1990 HONORS AND AWARDS Dr. Coligan was invited to present laboratory data seminars at the Center for Disease Control in Atlanta and the University of Leiden in Holland. He was also invited to make presentations at the MHC Workshop in Bavaria, West Germany, the FASEB meeting in New Orleans and the Workshop in Molecular Biology of MHC Genes in Italy. Dr. Kevin Holmes made an in invited presentation at the UCLA symposium on B-cell development. Duphaston Tablets Dr. Coligan continues to serve on the editorial boards of Molecular Immunology and Immunologic Research and during the past year was asked to serve on the editorial broad of Current Protocols in Immunology . Dr. Coligan was appointed to the Senior Executive Service. He was also appointed as Chairperson on the Program Committee for the Major Histocompatibility Complex Section of the American Association of Immunologists Annual Meeting. 4-7 DEPARTMENT OF HEALTH AND HUMAN SERVICES ■ PUBLIC HEALTH SERVICE NOTICE OP INTRAMURAL RESEARCH PROJECT Z01 AI 00169-13 BRB period covered 0ctober 1? 1989 to September 30, 1990 TITLE O r PROJECT (BO criBrtcrv'-s & *sj Hr% must fn on onm knt fc»rw»#" rnt boTtots ) Analysis of Murine and Human Transplantation Antigens and Genes PRINCIPAL INVESTIGATOR (Uil *>•' pronystKmt ptionn*' t»*-v» m« PnrKipt In v» $ ng I frv ; (Htmt W« /•Oortrcvy WO IniWTVt* »fWi«r>on) PI: John E. Coligan Branch Chief BRB, NIAID COOPERATING UNITS (K »nyj P. Kourilsky, Pasteur Institute; E. Long, LIG, NIAID, W. Biddison, NI, NINCDS, D. Margulies, LI, NIAID. LAB/BRANCH Biological Resources Branch Office of the Chief INSTITUTE ANO LOCATKD NIAID, NIH, Bethesda, MD 20892 TOTAL MAN- YEARS 1.2 PROFESSIONAL 1.0 CHECK APPROPRIATE BOXfES) D (a) Human subjects D (b) Human tissues S (c) Neither D (a1) Minors D (a2) Interviews SUMMARY OP WORK (Um l«otf»TJ unnducw} tyv Ck> not Ito—d fi>* nf*c* prov«fd ) Two different HLA-A2 Duphaston And Clomid genes were found to be expressed in cells from donor Q66; one is identical to HLA-A2.1 and the other is identical to HLA-A2.2F (Gin — » Arg at position 43, Val -» Leu at position 95, and Leu -» Trp at position 156). Studies with CTL generated from this donor demonstrated that a different CTL repertoire specific for HLA-A2 plus the Ml 55-73 peptide is generated in an individual that expresses both HLA-A2.1 and HLA-A2.2F compared to individuals who express HLA-A2.1 alone, and that the unique repertoire of T cell receptors can be selected by the presence of an HLA-A2 molecule with a single amino acid substitution at position 156. A peptide derived from o^ helical region of the H-2K b molecule,
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